Linked Life Data

2612729

Source:http://linkedlifedata.com/resource/pubmed/id/2612729

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-3-8
pubmed:abstractText
A sensitive avian growth hormone (GH) radioreceptor assay (RRA) was developed using recombinant chicken growth hormone (rcGH) and a membrane receptor preparation of chicken or turkey livers. The specific binding of 125I-labeled rcGH to a 47,800 X g pellet was 33-36% in a 16-20 h incubation period at 4 degrees C. Binding was time, temperature and pH dependent. Scatchard analysis indicated a single class of high affinity GH binding sites in chicken and turkey livers, with binding affinities of 1.03 X 10(10) liter/M and 1.11 X 10(10) liter/M, respectively, and corresponding binding capacities of 10.7 fmol and 21 fmol per mg protein. The sensitivity of the assay was 0.41 ng of rcGH. Intra- and inter-assay coefficients of variation were 5.3% and 9.7%, respectively. Bovine GH, ovine GH, and porcine GH competed effectively for the GH binding sites in chicken and turkey livers. Turkey prolactin (PRL) and porcine PRL showed little cross-reaction (less than 0.07%), while cross-reaction of ovine and bovine PRL was greater (less than 10%). Standard rcGH (0.5-30 ng) was added to sera from hypophysectomized chickens and turkeys (hypox sera) and to tissue culture medium and was measured quantitatively. Untreated medium (10-100 microliters) and hypox sera (5-40 microliters) did not inhibit rcGH binding. These studies report the existence of specific binding sites for avian GH in chicken and turkey liver and validate a sensitive RRA for measurement of bioactive GH in sera and tissue culture medium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-34
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Avian growth hormone receptor assay: use of chicken and turkey liver membranes.
pubmed:affiliation
U.S. Department of Agriculture, ARS, Avian Physiology Laboratory, Beltsville, MD 20705.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.