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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1990-2-9
|
| pubmed:abstractText |
Human pancreatic kallikrein (H.Panc.K.) was purified from human pancreas by ion exchange chromatography on DEAE-cellulose, affinity chromatographies on p-aminobenzamidine Sepharose 6B and aprotinin aminocellulofine, followed by gel filtration on Sephacryl S-200. The final preparation had a specific activity of 9.2 AU/A280 (AU; amidase unit for H-Pro-Phe-Arg-MCA) and its N-terminal sequence coincided with the reported sequence for H.Panc.K.. In HPLC (gel filtration), one symmetrical peak corresponding to a molecular weight of 48,000 was obtained. In SDS-PAGE without 2-mercaptoethanol (2-ME), one band corresponding to a molecular weight of 52,000 was obtained, but with 2-ME, 2 bands, 52,000 and 30,000, were obtained. Km value for MCA was 4.9 x 10(-2) mM. Proteinase inhibitor specificities of H.Panc.K. were the same as those of human urinary kallikrein (HUK) and hog pancreatic kallikrein (hog Panc.K.), while anti-HUK antibody inhibited the activities of H.Panc.K. and HUK, but not that of hog Panc.K.. From the analysis of affinity for concanavalin A (Con A) and erythroagglutinating phytohemagglutinin (E-PHA), the carbohydrate parts of H.Panc.K. are relatively rich in biantennary complex type sugar chains with bisecting GlcNAc compared with those of human salivary kallikrein (H.Saliv.K.) and HUK.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0065-2598
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
247B
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
177-82
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2610057-Amino Acid Sequence,
pubmed-meshheading:2610057-Chromatography, Affinity,
pubmed-meshheading:2610057-Chromatography, DEAE-Cellulose,
pubmed-meshheading:2610057-Chromatography, Gel,
pubmed-meshheading:2610057-Humans,
pubmed-meshheading:2610057-Isoenzymes,
pubmed-meshheading:2610057-Kallikreins,
pubmed-meshheading:2610057-Kinetics,
pubmed-meshheading:2610057-Molecular Sequence Data,
pubmed-meshheading:2610057-Molecular Weight,
pubmed-meshheading:2610057-Pancreas
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Characterization of human pancreatic kallikrein.
|
| pubmed:affiliation |
1st Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|