Linked Life Data

2607993

Source:http://linkedlifedata.com/resource/pubmed/id/2607993

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
242
pubmed:dateCreated
1990-2-13
pubmed:abstractText
Exfoliated buccal epithelial cells have been widely used in microbial adherence studies, but present a number of problems due to their variable nature and to contamination with indigenous bacteria. An adherence assay was developed using HeLa cell monolayers which were washed with buffer, or treated with saliva or serum to mimic buccal or crevicular epithelial cells, respectively. A total of eighteen strains of oral bacteria tested showed a low affinity for untreated HeLa cells, but most strains adhered in high numbers to saliva treated HeLa cells. A few strains, usually present in the gingival crevice, demonstrated a high affinity for serum treated HeLa cells. Thus, salivary and crevicular fluid components appear to be specifically implicated in the selective adherence and colonization of bacteria on oral surfaces.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0026-2633
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
An in vitro method to study the adherence of oral bacteria to HeLa cells.
pubmed:affiliation
Department of Oral Medicine and Pathology, University of Glasgow, Dental Hospital and School, Scotland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't