Linked Life Data

2603161

Source:http://linkedlifedata.com/resource/pubmed/id/2603161

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-2-8
pubmed:abstractText
Using isolated brush border membrane vesicles from the kidney of the winter flounder (Pseudopleuronectes americanus), we have studied the effect of cadmium on L-alanine transport. Pretreatment of vesicles with 0.1 mM Cd2+ resulted in inhibition of L-alanine uptake in the presence of a NaCl (but not KCl) gradient. Inhibition was due to a specific interaction with the sodium-alanine cotransport system and not a change in the driving forces for alanine transport, since Cd2+ did not affect sodium-dependent D-glucose uptake. The effect of Cd2+ on Na+-alanine cotransport showed mixed-type inhibition which is only partially reversible by EDTA. Cd2+ uptake itself was shown to be time and temperature dependent, resulting in binding to both sides of the membrane. No direct correlation was possible between inhibition of L-alanine transport and the amount of Cd2+ taken up by the membranes. Nevertheless, the striking time dependence of the effect of Cd2+ on sodium-dependent L-alanine uptake and the inability of EDTA to reverse the inhibitory action of Cd2+ suggest that Cd2+ inhibits Na+-alanine cotransport at the cytoplasmic side of the membrane.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
461-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Cadmium inhibition of L-alanine transport into renal brush border membrane vesicles isolated from the winter flounder (Pseudopleuronectes americanus).
pubmed:affiliation
Max-Planck-Institut für Systemphysiologie, Dortmund, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't