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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1990-2-1
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pubmed:abstractText |
DNA is subject to constant oxidative damage from endogenous oxidants. The oxidized DNA is continuously repaired and the oxidized bases are excreted in the urine. A simple routine analytical procedure is described for urinary 8-hydroxy-2'-deoxyguanosine, an oxidative DNA damage adduct, as an indicator of oxidative damage in humans and rodents. This adduct was purified from human urine and characterized. The described assay employs a series of solid-phase extraction steps that separate 8-hydroxy-2'-deoxyguanosine from other urinary constituents, followed by analysis by gradient reversed-phase HPLC coupled to a dual-electrode high-efficiency electrochemical detection system. Analysis of urine from three species by this method indicates that mice excrete approximately 3.3-fold more 8-hydroxy-2'-deoxyguanosine than humans (582 vs. 178 residues per cell per day), a result that supports the proposal that oxidative damage to DNA increases in proportion to species-specific basal metabolic rates.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-2519715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-2577733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-2648148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-2838476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3017148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3128794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3413108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3574469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3677321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3757945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3769133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-3789740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-4121880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-5573399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-6264426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-6351251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-6592579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-6701097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2602371-6888408
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9697-701
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2602371-Animals,
pubmed-meshheading:2602371-Biological Markers,
pubmed-meshheading:2602371-Chromatography, High Pressure Liquid,
pubmed-meshheading:2602371-DNA Damage,
pubmed-meshheading:2602371-Deoxyguanosine,
pubmed-meshheading:2602371-Humans,
pubmed-meshheading:2602371-Mice,
pubmed-meshheading:2602371-Oxidation-Reduction,
pubmed-meshheading:2602371-Rats,
pubmed-meshheading:2602371-Rats, Inbred F344,
pubmed-meshheading:2602371-Reference Values,
pubmed-meshheading:2602371-Species Specificity
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pubmed:year |
1989
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pubmed:articleTitle |
Urinary 8-hydroxy-2'-deoxyguanosine as a biological marker of in vivo oxidative DNA damage.
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pubmed:affiliation |
Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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