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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1978-7-15
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pubmed:abstractText |
Sepharose 4B column with antibody to tyrosine aminotransferase (E.C. 2.6.1.5) (TAT) covalently bound can selectively remove a specific fraction of TAT polysomes from rat liver homogenates. From the polysomes which was adsorbed by immunosorbent one may obtain mRNA containing a segment of poly-adenilate-rich residues, having sedimentation constant of 11--16S. This mRNA may program synthesis of the specific protein in a cell free system. Near 70% of the protein was synthesized in such a system, may react with the antibody to TAT.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0026-8984
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
260-6
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:26021-Animals,
pubmed-meshheading:26021-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:26021-Immunosorbent Techniques,
pubmed-meshheading:26021-Male,
pubmed-meshheading:26021-Polyribosomes,
pubmed-meshheading:26021-RNA, Messenger,
pubmed-meshheading:26021-Rats,
pubmed-meshheading:26021-Tyrosine Transaminase
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pubmed:articleTitle |
Extraction of tyrosine aminotransferase mRNA by polyribosome immunosorption on sepharose 4B.
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pubmed:publicationType |
Journal Article
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