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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-1-24
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pubmed:abstractText |
A cDNA clone encoding porcine brain natriuretic peptide (BNP) precursor was used to probe a canine genomic DNA library for homologous sequences. A unique clone was obtained (D1) which encoded a peptide homologous to porcine BNP. A 4.0 kB Hind III fragment of D1 containing the entire BNP precursor coding region was hybridized against blots of EcoRI digested human genomic DNA. A 7.0 kB hybridizing band was observed, which was subsequently subcloned and sequenced. This human clone, H1, also contained a sequence homologous to porcine BNP precursor. Transcription of the human BNP gene was confirmed by detection of BNP-specific sequences amplified from human atrial RNA by polymerase chain reaction. These results suggest that both BNP and ANP are present and function in a wide variety of mammalian species.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
650-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2597152-Amino Acid Sequence,
pubmed-meshheading:2597152-Animals,
pubmed-meshheading:2597152-Base Sequence,
pubmed-meshheading:2597152-Brain,
pubmed-meshheading:2597152-Cloning, Molecular,
pubmed-meshheading:2597152-DNA,
pubmed-meshheading:2597152-Dogs,
pubmed-meshheading:2597152-Gene Library,
pubmed-meshheading:2597152-Genes,
pubmed-meshheading:2597152-Humans,
pubmed-meshheading:2597152-Molecular Sequence Data,
pubmed-meshheading:2597152-Natriuretic Peptide, Brain,
pubmed-meshheading:2597152-Nerve Tissue Proteins,
pubmed-meshheading:2597152-Oligonucleotide Probes,
pubmed-meshheading:2597152-Polymerase Chain Reaction,
pubmed-meshheading:2597152-Protein Precursors,
pubmed-meshheading:2597152-Restriction Mapping,
pubmed-meshheading:2597152-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2597152-Swine
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pubmed:year |
1989
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pubmed:articleTitle |
Human and canine gene homologs of porcine brain natriuretic peptide.
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pubmed:affiliation |
California Biotechnology Inc., Mountain View 94043.
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pubmed:publicationType |
Journal Article,
Comparative Study
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