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rdf:type |
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lifeskim:mentions |
umls-concept:C0005919,
umls-concept:C0008405,
umls-concept:C0008564,
umls-concept:C0039654,
umls-concept:C0079411,
umls-concept:C0205214,
umls-concept:C0441712,
umls-concept:C0687342,
umls-concept:C0936012,
umls-concept:C1257740,
umls-concept:C1514468,
umls-concept:C1623117,
umls-concept:C2612755
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pubmed:issue |
2
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pubmed:dateCreated |
1990-1-19
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pubmed:abstractText |
A method for the rapid and quantitative separation of glycerophosphocholine, choline phosphate and choline upon ion-exchange columns is described. The method has been utilized to examine the stimulation of phosphatidylcholine breakdown in quiescent Swiss 3T3 cells in response to bombesin and 12-O-tetradecanoylphorbol 13-acetate (TPA). The stimulated generation of choline is shown to precede that of choline phosphate, with no effect upon glycerophosphocholine levels; but was attenuated in cells in which protein kinase C activity was down-regulated. The results thus suggest that stimulation of the cells with either bombesin or TPA activates phospholipase D-catalysed phosphatidylcholine breakdown by a common mechanism involving the activation of protein kinase C.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-1254572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-2543367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-2548481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-2821987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-2822028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-2842326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-2844277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-2987212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3032258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3117799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3365241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3384087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3422154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3558493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3566713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3597411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-3748188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-4284220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-4656795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-6089798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-6233972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-6244502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-6365172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-7085651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2597122-7306057
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
581-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2597122-Bombesin,
pubmed-meshheading:2597122-Cell Line,
pubmed-meshheading:2597122-Choline,
pubmed-meshheading:2597122-Chromatography, Ion Exchange,
pubmed-meshheading:2597122-Fibroblasts,
pubmed-meshheading:2597122-Glycerylphosphorylcholine,
pubmed-meshheading:2597122-Kinetics,
pubmed-meshheading:2597122-Phosphatidylcholines,
pubmed-meshheading:2597122-Phosphorylcholine,
pubmed-meshheading:2597122-Solubility,
pubmed-meshheading:2597122-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2597122-Tritium,
pubmed-meshheading:2597122-Water
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pubmed:year |
1989
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pubmed:articleTitle |
Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism.
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pubmed:affiliation |
Department of Biochemistry, University of Glasgow, Scotland, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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