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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1990-1-25
|
| pubmed:abstractText |
A simple, highly sensitive and specific high-performance liquid chromatographic method that uses a coulometric detector for the simultaneous assay of haloperidol and reduced haloperidol in human plasma has been developed, using bromoperidol as the internal standard. A reversed phase C8 5-microns column (25 x 0.46 cm) and a mobile phase of phosphate buffer (pH 7.0), acetonitrile, and methanol (40:20:40 vol/vol) are used for separating the analytes. The analytes are extracted from alkalinized plasma using a mixture of pentane and isopropanol (95:5 vol/vol) and purified by back extraction into a perchloric acid solution. Teflon tubes with screw caps are used throughout the extraction work. The compounds are oxidized at a potential of +0.90 V against a Ag/AgCl reference electrode. The detection limit of the assay is 50 ng/L using 1 mL of plasma. The average interassay coefficient of variation for samples of concentration 1-40 micrograms/L is approximately 8%. Possible drug interferences in the assay have been studied. The absolute recovery of the method is approximately 80%. The assay has been validated by quantitating 150 schizophrenic patients' samples.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0163-4356
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
701-7
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2595753-Chromatography, High Pressure Liquid,
pubmed-meshheading:2595753-Chromatography, Liquid,
pubmed-meshheading:2595753-Electrochemistry,
pubmed-meshheading:2595753-Haloperidol,
pubmed-meshheading:2595753-Humans,
pubmed-meshheading:2595753-Hydrogen-Ion Concentration,
pubmed-meshheading:2595753-Oxidation-Reduction,
pubmed-meshheading:2595753-Psychotropic Drugs,
pubmed-meshheading:2595753-Spectrophotometry, Ultraviolet
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
An improved sensitive assay for simultaneous determination of plasma haloperidol and reduced haloperidol levels by liquid chromatography using a coulometric detector.
|
| pubmed:affiliation |
Department of Psychiatry, University of Michigan Medical School, Ann Arbor 48109-0656.
|
| pubmed:publicationType |
Journal Article
|