Linked Life Data

2591475

Source:http://linkedlifedata.com/resource/pubmed/id/2591475

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-1-12
pubmed:abstractText
A large conductance Ca2+-activated K+ channel from rabbit aorta was incorporated into planar lipid bilayers. This channel had a conductance of 337 +/- 7 pS in symmetrical 250 mM KCl solutions and had a Na+/K+ permeability ratio of less than 0.04. In asymmetrical solutions containing 300 mM KCl cis (intracellular), 100 mM KCl trans (extracellular) or 100 mM KCl cis 500 mM KCl trans, the reversal potentials for the channel were -30 and +46 mV, respectively. This channel possessed voltage-dependent activation and cis (intracellular) Ca2+ sensitivity. Cromakalim (50 nM) added to the trans side of the bilayer significantly increased the Popen by 56% from 0.09 +/- 0.01 to 0.14 +/- 0.01 (P less than 0.01) at -40 mV without altering the single channel conductance. This effect was dose-dependent, increasing at higher cromakalim concentrations. The primary effect of cromakalim was to decrease the tau slow of the channel closed state from 266 +/- 32 to 147 +/- 17 ms which is sufficient to account for the increase in Popen of the channel in the presence of cromakalim.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-10
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
A Ca2+-activated K+ channel from rabbit aorta: modulation by cromakalim.
pubmed:affiliation
Department of Pharmacology, University of Miami School of Medicine, FL 33101.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.