Linked Life Data

2589058

Source:http://linkedlifedata.com/resource/pubmed/id/2589058

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1990-1-3
pubmed:abstractText
A new procedure is described for determination of sodium and potassium in rat lenses with atomic absorption spectrophotometry. Samples were prepared by soaking of the ground or the intact lens in deionized water. For potassium determinations HNO3 dissolution was also tried. Deionized water or CsCl aqueous solution was adopted for dilution for measurement. At absorbances up to 1.0 absorbance unit only measurements on HNO3 dissolved lenses diluted in deionized water were compatible with a linear absorbance function. At absorbances below 0.2-0.3 absorbance units all combinations were in agreement with a linear absorbance function. Grinding of the lens was found to increase the variability but did not change the level. HNO3 dissolution did not increase the level. Dilution with CsCl increased the sensitivity and decreased the variability. A schedule is proposed for determination of sodium and potassium in which lens samples are obtained by soaking in deionized water. Aqueous solution of CsCl is suggested for dilution.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0001-639X
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
582-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Determination of Na and K in the rat lens by atomic absorption spectrophotometry.
pubmed:affiliation
Department of Medical Biophysics, Karolinska Institutet, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't