Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1990-1-9
pubmed:abstractText
The presence of the human immunodeficiency virus (HIV) genome was investigated by applying in situ hybridisation techniques to peripheral blood mononuclear cells (PBMCs). Twenty asymptomatic anti-HIV seronegative homosexual men were the subjects of our study. The cells were hybridised with: (1) an SP 64 plasmid containing the nine-kilobase SstI-SstI viral insert from the lambda BH 10 recombinant clone; this can recognise both viral RNA and proviral DNA, and (2) with a pA01 plasmid containing HBV DNA genome. The DNA probes were modified by inserting an antigenic sulfone group in the cytosine moieties and the visualisation was performed by a double antibody immunohistochemical reaction. In two subjects both the HIV genome and HBV DNA were detected whereas another two subjects were positive for HBV DNA and for the HIV genome respectively. Thus people who are seronegative for anti-HIV specific antibodies may be infected with HIV.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-2410981, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-2448875, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-2716797, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-2887886, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-2981429, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3063757, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3163848, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3277271, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3277274, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3299092, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3336784, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3422337, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3461456, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-3497224, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-4045437, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-6095086, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-6169587, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-6260871, http://linkedlifedata.com/resource/pubmed/commentcorrection/2583712-6928684
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0266-4348
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
293-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Detection of HIV genome in HIV antibody negative men.
pubmed:affiliation
Institute of Infectious Diseases, University of Rome La Sapienza, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't