|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
1985-6-25
|
|
pubmed:abstractText |
We have shown previously that a beta-globin RNA-deficient beta zero-thalassemia is caused by a single base-pair deletion in codon 44 of the human beta-globin gene1. The lack of beta-globin RNA in erythroid cells of these affected individuals is due to extreme beta-globin RNA instability (t 1/2 = 30 min)2. We have further investigated the mechanism of this extreme lability by transiently expressing the beta zero-thalassemic allele in HeLa cells and assaying the stability of the beta-globin RNA that is produced. Surprisingly, the beta zero-thalassemic RNA is much more stable in HeLa cells than in bone marrow cells. Apparently, developing erythroid cells have a mechanism for turning over this thalassemic RNA while cervical carcinoma cells do not. We also have assayed the stability of RNA derived from in vitro-mutagenized beta-globin genes. In HeLa cells, beta-globin RNAs harboring deletions in and around the translation initiation codon accumulate to steady-state levels that are similar to the level of normal beta-globin RNA.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-206891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-390497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-4524649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-4796595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-487427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6101206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6152853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6186918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6272205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6292840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6933479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-6937265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-7151176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-7431395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2582366-853064
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
25
|
|
pubmed:volume |
13
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2855-67
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2582366-Alleles,
pubmed-meshheading:2582366-Animals,
pubmed-meshheading:2582366-Base Sequence,
pubmed-meshheading:2582366-Bone Marrow,
pubmed-meshheading:2582366-Globins,
pubmed-meshheading:2582366-Half-Life,
pubmed-meshheading:2582366-HeLa Cells,
pubmed-meshheading:2582366-Humans,
pubmed-meshheading:2582366-Mice,
pubmed-meshheading:2582366-RNA,
pubmed-meshheading:2582366-Thalassemia
|
|
pubmed:year |
1985
|
|
pubmed:articleTitle |
A beta zero-thalassemic beta-globin RNA that is labile in bone marrow cells is relatively stable in HeLa cells.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
