Linked Life Data

2582102

Source:http://linkedlifedata.com/resource/pubmed/id/2582102

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-6-28
pubmed:abstractText
Substance P (SP) is widely distributed in the spinal cord and has been implicated as a neurotransmitter in several spinal cord neuronal systems. To investigate SP receptors in the spinal cord, 125I-Bolton-Hunter-SP (125I-BH-SP) was used to identify and characterize spinal cord binding sites for the peptide. The binding of 125I-BH-SP had the following characteristics: high affinity; time, temperature, and membrane concentration dependent; reversible; and saturable. The IC50 of SP in whole spinal cord was 0.46 nM as compared with 0.95, 60, and 150 nM for physalaemin, eledoisin, and kassinin. Four putative antagonists of SP were less than 0.0001 times as potent as SP in inhibiting 125I-BH-SP binding. IC50s were 5, 7.5, 7.0, and 45 microM for D-Pro2, D-Trp7,9-SP; D-Pro2, D-Phe7, D-Trp9-SP; D-Arg1, D-Pro2, D-Trp7,9, Leu11-SP; and D-Pro4, D-Trp7,9,10-SP(4-11), respectively. The lumbosacral section bound 3 times more SP than the cervical and thoracic sections, although IC50 for the cervical section was 0.06 of that for the lumbosacral and thoracic sections. The data suggest more than one class of binding site for SP in the spinal cord and indicate a direct role for SP in spinal cord functions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1293-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Characterization and segmental distribution of 125I-Bolton-Hunter-labeled substance P binding sites in rat spinal cord.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.