| pubmed-article:2582059 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2582059 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2582059 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:2582059 | lifeskim:mentions | umls-concept:C0021745 | lld:lifeskim |
| pubmed-article:2582059 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:2582059 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:2582059 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2582059 | pubmed:dateCreated | 1985-7-12 | lld:pubmed |
| pubmed-article:2582059 | pubmed:abstractText | Two stable hybridoma cell lines secreting specific antibodies against human gamma interferon (HuIFN-gamma) were established. Both monoclonal antibodies (designated as MD-1 and MD-2) belong to the IgG1/kappa subclass and neutralize the antiviral activity of natural and recombinant DNA derived HuIFN-gamma (nHuIFN-gamma and rHuIFN-gamma respectively), although MD-1 is far more effective than MD-2. MD-1 and MD-2 recognize different epitopes and do not compete with each other in binding to HuIFN-gamma as concluded from competition assays. In a 'Western' blot, both antibodies reacted with the 20 kDa and 25 kDa polypeptides present in nHuIFN-gamma preparations. A sandwich enzyme immunoassay using microtiter plates coated with unlabeled MD-2 was developed. Biotinylated MD-1 was used as the second antibody. Bound MD-1 was detected by an avidin/alkaline phosphatase enzyme reaction. This immunoassay is highly specific and as sensitive as a bioassay. A radioimmunoassay using MD-2 coated on polystyrene balls and 125I-labeled MD-1 as the second antibody showed a sensitivity comparable to that of the enzyme immunoassay. | lld:pubmed |
| pubmed-article:2582059 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2582059 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2582059 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2582059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2582059 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2582059 | pubmed:month | May | lld:pubmed |
| pubmed-article:2582059 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:2582059 | pubmed:author | pubmed-author:SchellekensHH | lld:pubmed |
| pubmed-article:2582059 | pubmed:author | pubmed-author:Van der... | lld:pubmed |
| pubmed-article:2582059 | pubmed:author | pubmed-author:DubbeldMM | lld:pubmed |
| pubmed-article:2582059 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2582059 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:2582059 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:2582059 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2582059 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2582059 | pubmed:pagination | 293-305 | lld:pubmed |
| pubmed-article:2582059 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:2582059 | pubmed:meshHeading | pubmed-meshheading:2582059-... | lld:pubmed |
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| pubmed-article:2582059 | pubmed:meshHeading | pubmed-meshheading:2582059-... | lld:pubmed |
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| pubmed-article:2582059 | pubmed:meshHeading | pubmed-meshheading:2582059-... | lld:pubmed |
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| pubmed-article:2582059 | pubmed:meshHeading | pubmed-meshheading:2582059-... | lld:pubmed |
| pubmed-article:2582059 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2582059 | pubmed:articleTitle | Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA. | lld:pubmed |
| pubmed-article:2582059 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2582059 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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