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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-7-17
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pubmed:abstractText |
Monoclonal antibodies (MAbs) to rat liver cytochromes P-450 have previously been used for successful immunopurification of cytochromes P-450 from animal tissues. We now report application of this MAb-based immunopurification technique to the human lymphoblastoid AHH-1 cell line. Immunopurification carried out with 3 different MAbs each yielded a 45-kDa polypeptide. The purified protein contains an MAb-specific epitope present on cytochromes P-450, and may therefore be a human cytochrome P-450.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
67-70
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2581816-Animals,
pubmed-meshheading:2581816-Antibodies, Monoclonal,
pubmed-meshheading:2581816-Antibody Specificity,
pubmed-meshheading:2581816-Cell Line,
pubmed-meshheading:2581816-Cytochrome P-450 Enzyme System,
pubmed-meshheading:2581816-Enzyme Induction,
pubmed-meshheading:2581816-Epitopes,
pubmed-meshheading:2581816-Humans,
pubmed-meshheading:2581816-Immunologic Techniques,
pubmed-meshheading:2581816-Liver,
pubmed-meshheading:2581816-Lymphocytes,
pubmed-meshheading:2581816-Methylcholanthrene,
pubmed-meshheading:2581816-Phenobarbital,
pubmed-meshheading:2581816-Rats
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pubmed:year |
1985
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pubmed:articleTitle |
Monoclonal antibodies to cytochrome P-450 immunopurify a 45-kDa protein from a human lymphoblastoid cell line.
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pubmed:publicationType |
Journal Article
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