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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1985-4-5
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pubmed:abstractText |
Ion transport in human erythrocytes was studied by 23Na and 39K NMR with an anionic paramagnetic shift reagent, Dy(P3O10)2(7-). The intra- and extracellular 23Na and 39K NMR signals were well separated (over 10 ppm) at 5 mM concentration of the shift reagent. The NMR visibility of the intracellular Na+ and K+ was determined to be 100% in human and duck erythrocytes. The intracellular ion concentrations were 8.1 +/- 0.8 mM Na+ (n = 7) and 110 +/- 12 mM K+ (n = 4) for fresh human erythrocytes. The ouabain-sensitive net Na+ efflux was 1.75 +/- 0.08 mmol/hr per liter of cells at 37 degrees C (n = 3). The gramicidin-induced ion transport in human erythrocytes was also studied by 23Na and 39K NMR or by simultaneous measurements of 23Na NMR and a K+-selective electrode. The time courses of the Na+ and K+ transport induced by the ionophore were biphasic. The initial rapid fluxes were due to an exchange of Na+ for K+, which were found to occur with a 1:1 stoichiometry. The subsequent slow components were the net Na+ and K+ effluxes rate-limited by the Cl- permeability and accompanied by a reduction in cell volume. The Cl- permeability determined from the NMR measurements of these slow fluxes was 3.2 +/- 0.5 X 10(-8) cm/sec at 25 degrees C (n = 4).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-129563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-130181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-13417139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-4541139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-5407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-5914742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6074969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6174981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6244501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6351054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6378069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6604460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6615821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6720941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6825412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6833998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-6870815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2579385-874904
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1099-103
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1985
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pubmed:articleTitle |
23Na and 39K NMR studies of ion transport in human erythrocytes.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, Non-P.H.S.
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