rdf:type |
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lifeskim:mentions |
|
pubmed:issue |
3
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pubmed:dateCreated |
1985-4-24
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pubmed:abstractText |
Three monoclonal antibodies selected for their recognition of parts of the T3-T cell receptor complex on human T lymphocytes were found to induce an increase in cytoplasmic free Ca2+ (Ca2+i) in the T cell leukemia line HPB-ALL as measured by Quin2 fluorescence. These reagents are directed against T3 (OKT3), a nonvariable T3-associated structure (WT-31) and the variable region of the T3-associated antigen receptor (T40/25) of this cell line. The rise in Ca2+i was dependent on the presence of extracellular Ca2+, occurred within 30 sec of stimulation, and was sustained for at least 10 min. Fab fragments of OKT3 also caused a rapid increase in Ca2+i, indicating that cross-linking is not necessary to induce a Ca2+ response. Alterations in plasma membrane potential and La3+ blocked the Ca2+ influx induced by OKT3 and T40/25. These data suggest that the T3-T cell receptor complex of human T lymphocytes may be an antigen-regulated Ca2+ channel.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Lanthanum,
http://linkedlifedata.com/resource/pubmed/chemical/Quin2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
pubmed:issn |
0092-8674
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
583-90
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2578888-Aminoquinolines,
pubmed-meshheading:2578888-Antibodies, Monoclonal,
pubmed-meshheading:2578888-Calcium,
pubmed-meshheading:2578888-Cell Line,
pubmed-meshheading:2578888-Egtazic Acid,
pubmed-meshheading:2578888-Fluorescent Dyes,
pubmed-meshheading:2578888-Humans,
pubmed-meshheading:2578888-Ion Channels,
pubmed-meshheading:2578888-Lanthanum,
pubmed-meshheading:2578888-Leukemia,
pubmed-meshheading:2578888-Membrane Potentials,
pubmed-meshheading:2578888-Receptors, Antigen, T-Cell,
pubmed-meshheading:2578888-Spectrometry, Fluorescence,
pubmed-meshheading:2578888-T-Lymphocytes
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pubmed:year |
1985
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pubmed:articleTitle |
Stimulation of the T3-T cell receptor complex induces a membrane-potential-sensitive calcium influx.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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