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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-5-11
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pubmed:abstractText |
Neurospora crassa wild-type is almost unable to grow on glutamine as sole nitrogen and carbon source but a GDH-; GS +/- double mutant strain, lacking NADP-dependent glutamate dehydrogenase and partially lacking glutamine synthetase did grow. Under these conditions, the double mutant had a higher chemical energy content than the wild-type. Enzyme assays and labelling experiments with glutamine indicated that in the double mutant glutamine was degraded to ammonium and to carbon skeletons by glutamate synthase, the catabolic (NADH-dependent) glutamate dehydrogenase and the glutamine transaminase-omega-amidase pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1287
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
135
|
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2699-707
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2576659-Amino Acids,
pubmed-meshheading:2576659-Ammonia,
pubmed-meshheading:2576659-Carbon,
pubmed-meshheading:2576659-Energy Metabolism,
pubmed-meshheading:2576659-Glutamate Dehydrogenase,
pubmed-meshheading:2576659-Glutamate Synthase,
pubmed-meshheading:2576659-Glutamate-Ammonia Ligase,
pubmed-meshheading:2576659-Glutamine,
pubmed-meshheading:2576659-Mutation,
pubmed-meshheading:2576659-Neurospora,
pubmed-meshheading:2576659-Neurospora crassa,
pubmed-meshheading:2576659-Nitrogen,
pubmed-meshheading:2576659-Transaminases
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pubmed:year |
1989
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pubmed:articleTitle |
Glutamine assimilation pathways in Neurospora crassa growing on glutamine as sole nitrogen and carbon source.
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pubmed:affiliation |
Departamento de Ecología Molecular, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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