Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-5-8
pubmed:abstractText
We have studied the expression of four human homeobox genes representative of four different clusters (i.e., HOX-1, HOX-2, HOX-3 and HOX-5) in the embryonal carcinoma (EC) cell line NT2/D1. Following treatment with retinoic acid (RA), these cells differentiate into several cell types, including neurons, and steadily accumulate polyadenylated transcripts derived from the genes in a period ranging from 18 hr to 14 days of RA treatment. The sizes of major transcripts in differentiated EC cells coincide with those previously detected by the same probes in human embryos. Nuclear run-on transcriptional analysis showed no difference in the transcription rate of the four homeobox genes in differentiated vs. undifferentiated EC cells. Inhibition of protein synthesis by 5-18 hr of treatment of undifferentiated cells with cycloeximide causes accumulation of some homeobox transcripts at levels comparable to those observed after 18 hr of RA induction, although it does not cause superinduction in fully differentiated cells. These data suggest that the activation of homeobox gene expression in RA-induced EC cells is controlled, at least in part, by posttranscriptional mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1040-452X
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-15
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2576519-Amino Acid Sequence, pubmed-meshheading:2576519-Animals, pubmed-meshheading:2576519-Base Sequence, pubmed-meshheading:2576519-Cell Differentiation, pubmed-meshheading:2576519-DNA, pubmed-meshheading:2576519-DNA Probes, pubmed-meshheading:2576519-Embryonal Carcinoma Stem Cells, pubmed-meshheading:2576519-Gene Expression Regulation, pubmed-meshheading:2576519-Genes, Homeobox, pubmed-meshheading:2576519-Humans, pubmed-meshheading:2576519-Molecular Sequence Data, pubmed-meshheading:2576519-Neoplastic Stem Cells, pubmed-meshheading:2576519-RNA Processing, Post-Transcriptional, pubmed-meshheading:2576519-Sequence Homology, Nucleic Acid, pubmed-meshheading:2576519-Transcription, Genetic, pubmed-meshheading:2576519-Tretinoin, pubmed-meshheading:2576519-Tumor Cells, Cultured
pubmed:year
1989
pubmed:articleTitle
Posttranscriptional control of human homeobox gene expression in induced NTERA-2 embryonal carcinoma cells.
pubmed:affiliation
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't