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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-1-19
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pubmed:abstractText |
N epsilon-(gamma-Glutamyl)-lysine isodipeptide was detected in a protein-free fraction of Chinese-hamster ovary cells and their culture fluid by using radioactive lysine as a tracer. The identity of the isodipeptide was established by its separation on ion-exchange chromatography, analysis by h.p.l.c. after derivatization, recovery of lysine after acidic hydrolysis or after cleavage by a specific enzyme, namely gamma-glutamylamine cyclotransferase. The amount of isodipeptide was raised (460 pmol/10(7) cells and 61 pmol/ml of culture fluid were observed as highest values) as the cell density increased. Effects of inhibitors of intracellular protein degradation have shown that the isodipeptide derives from cross-linking N epsilon-(gamma-glutamyl)-lysine bonds formed by tissue transglutaminase. Estimated half-life values of cross-linked proteins were about 3 h. gamma-Glutamylamine cyclotransferase, which may split the isodipeptide formed during the continuous turnover of cross-linked proteins, was also found in Chinese-hamster ovary cells. Isodipeptide may have been accumulated when either its generated amount is beyond the capacity of gamma-glutamylamine cyclotransferase or it is generated in cell compartments where this enzyme is not present.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-12508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2414284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2824267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2859286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2869032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2890537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2892533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2893644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2900023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-2903774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-3007210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-3170689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-3240338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-3304137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-3922436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-42494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-4604628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-5794235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6102569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6104988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6105840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6117008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6117852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6153122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6160118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6200757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6396315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6438442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6786352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-6934510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-73346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-7387633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-7422874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574570-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dipeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Leupeptins,
http://linkedlifedata.com/resource/pubmed/chemical/Methylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transglutaminases,
http://linkedlifedata.com/resource/pubmed/chemical/chymostatin,
http://linkedlifedata.com/resource/pubmed/chemical/epsilon-(gamma-glutamyl)-lysine,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Glutamylcyclotransferase,
http://linkedlifedata.com/resource/pubmed/chemical/leupeptin,
http://linkedlifedata.com/resource/pubmed/chemical/methylamine
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
843-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2574570-Animals,
pubmed-meshheading:2574570-Cell Division,
pubmed-meshheading:2574570-Cell Line,
pubmed-meshheading:2574570-Chromatography, Ion Exchange,
pubmed-meshheading:2574570-Cricetinae,
pubmed-meshheading:2574570-Cricetulus,
pubmed-meshheading:2574570-Dipeptides,
pubmed-meshheading:2574570-Leupeptins,
pubmed-meshheading:2574570-Methylamines,
pubmed-meshheading:2574570-Oligopeptides,
pubmed-meshheading:2574570-Proteins,
pubmed-meshheading:2574570-Transglutaminases,
pubmed-meshheading:2574570-gamma-Glutamylcyclotransferase
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pubmed:year |
1989
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pubmed:articleTitle |
Formation of N epsilon-(gamma-glutamyl)-lysine isodipeptide in Chinese-hamster ovary cells.
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pubmed:affiliation |
Department of Biochemistry, University School of Medicine, Debrecen, Hungary.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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