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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1990-1-19
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pubmed:abstractText |
Most developmentally regulated epitopes identified on embryonal carcinoma cells and murine preimplantation embryos are associated with a glycoprotein-bound large glycan called embryoglycan. To prepare monoclonal antibodies recognizing other, less immunogenic stage-specific embryonic epitopes, we used embryoglycan-negative embryonal carcinoma cells P19XT.1.1 as immunogen. One monoclonal antibody prepared by this strategy was found to react specifically with mouse embryonal carcinoma and embryo-derived stem cell lines. The target epitope, TEC-4, was found to be expressed on eggs and two-cell embryos but was undetectable on later stages of mouse embryos and adult mouse tissues. NaDodSO4/PAGE of immunoaffinity-isolated antigen revealed that TEC-4 epitope is associated with glycoproteins of apparent Mr 120,000 and 240,000. The epitope was resistant to oxidation by sodium periodate and to digestion by endoglycosidase F but was sensitive to treatment with protein-denaturing agents and proteases, which suggested that the epitope is located in the protein moiety of the molecule. In the course of retinoic acid-induced differentiation of embryonal carcinoma cells the epitope disappeared before the onset of morphological differentiation. The combined data indicate that TEC-4 is an unusual stage-specific embryonic antigen that may be amenable to direct genetic analysis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2429884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2441394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2443254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2449911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2458292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2464500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2581941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-281705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2860973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-2918859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-3277983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-3283939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-3477527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-4160044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-4361679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-558524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6183004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6535637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-66184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6656766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6692975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6694356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6787139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6950406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-6967725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-7056443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-7095898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-7107698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2574458-7264323
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD15,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/embryoglycan
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9337-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2574458-Animals,
pubmed-meshheading:2574458-Antibodies, Monoclonal,
pubmed-meshheading:2574458-Antigens, CD15,
pubmed-meshheading:2574458-Antigens, Neoplasm,
pubmed-meshheading:2574458-Cell Differentiation,
pubmed-meshheading:2574458-Cell Line,
pubmed-meshheading:2574458-Cytotoxicity, Immunologic,
pubmed-meshheading:2574458-Fluorescent Antibody Technique,
pubmed-meshheading:2574458-Gene Expression,
pubmed-meshheading:2574458-Glycolipids,
pubmed-meshheading:2574458-Mice,
pubmed-meshheading:2574458-Molecular Weight,
pubmed-meshheading:2574458-Polysaccharides,
pubmed-meshheading:2574458-Teratoma,
pubmed-meshheading:2574458-Tretinoin,
pubmed-meshheading:2574458-Tumor Cells, Cultured,
pubmed-meshheading:2574458-Tumor Markers, Biological
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pubmed:year |
1989
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pubmed:articleTitle |
Unusual stage-specific embryonic antigen (TEC-4) defined by a monoclonal antibody to embryonal carcinoma cells defective in the expression of embryoglycan.
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pubmed:affiliation |
Department of Developmental Genetics, Czechoslovak Academy of Sciences, Prague.
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pubmed:publicationType |
Journal Article
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