Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1989-10-20
|
pubmed:abstractText |
The physiologic function of the monocyte transglutaminases is not known. In this study, we detected Factor XIII A-subunit antigen and "tissue" transglutaminase antigen in human monocytes by polyacrylamide gel electrophoresis and immunoblotting techniques. Flow cytometric analysis demonstrated that 27% and 49% of the total Factor XIII antigen in monocytes and human peritoneal macrophages, respectively, are expressed on the surface of the cells. Monocytes maintained in culture for 8 days had a 4-fold increase in Factor XIIIa activity and a 3.2-fold increase in the amount of Factor XIII antigen/mg cell protein. However, there was no increase in the "tissue" transglutaminase activity or antigen levels in cultured monocytes. In addition, we identified a Factor XIII deficient individual who does not express Factor XIII activity or antigen in plasma, platelets, monocytes, lymphocytes or erythrocytes. Intact monocytes from normal donors were able to cross-link fibrin formed in the plasma from the Factor XIII deficient individual. This suggests that transglutaminase activity expressed by peripheral blood monocytes may play a physiologic role in cross-linking fibrin during blood clotting or inflammation.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0049-3848
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
55
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
57-68
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2571199-Antigens, Surface,
pubmed-meshheading:2571199-Blood Platelets,
pubmed-meshheading:2571199-Cells, Cultured,
pubmed-meshheading:2571199-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2571199-Erythrocytes,
pubmed-meshheading:2571199-Factor XIII Deficiency,
pubmed-meshheading:2571199-Fibrin,
pubmed-meshheading:2571199-Humans,
pubmed-meshheading:2571199-Immunoblotting,
pubmed-meshheading:2571199-Lymphocytes,
pubmed-meshheading:2571199-Macrophages,
pubmed-meshheading:2571199-Monocytes,
pubmed-meshheading:2571199-Transglutaminases
|
pubmed:year |
1989
|
pubmed:articleTitle |
Human mononuclear phagocyte transglutaminase activity cross-links fibrin.
|
pubmed:affiliation |
Department of Medicine, Veterans Administration, Durham, North Carolina.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|