Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-9-21
pubmed:abstractText
Plasmin resulted in increased neutrophil adherence to cultured ovine pulmonary artery endothelial cell monolayers in a concentration-dependent manner (10(-12)-10(-7) M). The adherence response increased fivefold above baseline within 60 min after addition of plasmin (10(-8) M) and the response persisted up to 30 min after removal of plasmin. The neutrophil adherence was mediated by the action of plasmin on neutrophils rather than endothelial cells. The response was the result of an increase in functional activity of CD18 neutrophil cell surface adhesive glycoprotein. Neutrophil adherence was inhibited by pretreatment of neutrophils with MAbs IB4 and 60.3 targeted against the beta chain of the CD18, whereas control isotypic MAb 60.5 against HLA class I antigen had no effect. The plasmin catalytic site was not involved in the response. Lys-plasminogen had reduced adherence-promoting activity relative to plasmin, whereas glu-plasminogen had no effect. Elastase-derived plasminogen fragments corresponding to kringle 1+2+3 and kringle 4 (both of which contained the lysine-binding sites) possessed neutrophil adherence-promoting activities similar to plasmin, whereas miniplasminogen (which contains the catalytic site but no lysine-binding sites) had minimal effect, indicating the involvement of lysine-binding sites in the response. Blocking lysine-binding sites of plasmin and elastase-derived plasminogen fragments with tranexamic acid (IC50 of 5 mM) inhibited neutrophil adherence. A monospecific polyclonal antibody against the lysine-binding sites also reduced the neutrophil adherence-promoting activity of plasmin. The results indicate that plasmin induces neutrophil adherence to the endothelium and that the effect is mediated by lysine-binding sites on plasmin.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-2416819, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-2829380, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-2935966, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-2936810, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-2958480, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-2960990, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3013942, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3023400, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3086385, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3099830, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3156149, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3158094, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3160389, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3168176, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3278004, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3279059, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3417412, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3486903, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3553213, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3555290, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3745161, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3877078, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-3920216, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-4005427, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-4077977, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-4268896, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6090507, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-621200, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6225125, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6231920, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6348809, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6436212, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6480827, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6501563, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6772443, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6793589, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6833255, http://linkedlifedata.com/resource/pubmed/commentcorrection/2569479-6915938
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
793-801
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Role of catalytic and lysine-binding sites in plasmin-induced neutrophil adherence to endothelium.
pubmed:affiliation
Department of Physiology, Albany Medical College of Union University, New York 12208.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't