Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1989-8-18
|
| pubmed:abstractText |
We demonstrate here that the CD44 molecule, which mediates lymphocyte adhesion to high endothelial venules (HEV), is also involved in the delivery of an activation signal to the T cell. We have produced a CD44 mAb (H90) which is able to block the binding of lymphocytes to high endothelial venules. H90 had no effect on [3H]TdR incorporation of whole PBL stimulated by lectins, allogeneic cells, or CD3 mAb in the soluble phase; in contrast, it strongly increased [3H]TdR incorporation of PBL stimulated by CD2 pairs of mAb or by CD3 mAb linked to the plastic culture plates, when purified T cells were used, H90 mAb could efficiently induce them to proliferate after a primary signal of activation delivered via cross-linked CD3 or via CD2, an effect mediated by Il-2 synthesis and Il-2R expression. Thus, the effect of H90 mAb resembles the mitogenic effect of CD28 "9.3" mAb. However, several results show that CD28 and CD44 mediate different signals to the T cells: i) in contrast to CD28 mAb, CD44 mAb cannot complement the signal delivered by a soluble CD3 mAb, lectins, or PMA; ii) CD44 mAb, at the difference of CD28 mAb, cannot induce CD3+ thymocytes to proliferate in conjunction with a first signal provided via cross-linked CD3 or via CD2; iii) F(ab) fragments of H90 were efficient, whereas divalent fragments of CD29 9.3 mAb are required to produce activation signals; and iv) CD44 and CD28 mAb produce a very strong synergistic effect on T cell proliferation. These results fit with previous ones showing that endothelial cells can play the role of accessory cell in T cell activation and that a hierarchy of signaling can be delivered to T cells via CD3 and CD2.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD27,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
143
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
798-801
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2568380-Adjuvants, Immunologic,
pubmed-meshheading:2568380-Animals,
pubmed-meshheading:2568380-Antibodies, Monoclonal,
pubmed-meshheading:2568380-Antigens, CD2,
pubmed-meshheading:2568380-Antigens, CD27,
pubmed-meshheading:2568380-Antigens, CD3,
pubmed-meshheading:2568380-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2568380-Antigens, Surface,
pubmed-meshheading:2568380-Binding, Competitive,
pubmed-meshheading:2568380-Binding Sites, Antibody,
pubmed-meshheading:2568380-Cell Adhesion Molecules,
pubmed-meshheading:2568380-Endothelium, Lymphatic,
pubmed-meshheading:2568380-Humans,
pubmed-meshheading:2568380-Lymphocyte Activation,
pubmed-meshheading:2568380-Mice,
pubmed-meshheading:2568380-Molecular Weight,
pubmed-meshheading:2568380-Receptors, Antigen, T-Cell,
pubmed-meshheading:2568380-Receptors, Immunologic,
pubmed-meshheading:2568380-Receptors, Lymphocyte Homing,
pubmed-meshheading:2568380-T-Lymphocytes
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
CD44 contributes to T cell activation.
|
| pubmed:affiliation |
Laboratoire d'Immunologie des Tumeurs de l'Enfant, Institut Gustave-Roussy, Villejuif, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|