Linked Life Data

2567994

Source:http://linkedlifedata.com/resource/pubmed/id/2567994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1989-8-10
pubmed:abstractText
We have shown previously that reserpine is an effective "modulator" of P-glycoprotein-associated multidrug resistance (MDR). In addition to enhancing drug cytotoxicity in our multidrug-resistant human leukemia cell line, CEM/VLB100, reserpine strongly competes with a photoactivatible analog of vinblastine, N-(p-azido-3-[125I]iodosalicyl)-N'-(beta-aminoethyl)vindesine, for binding to P-glycoprotein. We also demonstrated previously that there are three substructural domains present in many compounds that modulate P-glycoprotein-associated MDR: a basic nitrogen atom and two planar aromatic rings. In the present study, we wished to test more rigorously the hypothesis that not only are these domains necessary for modulators of MDR but also they must exist in an appropriate conformation. Reserpine is a modulator of MDR in which these domains are present in a well-defined conformation. Accordingly, we prepared eight compounds that vary the spatial orientation of these domains, using either naturally occurring reserpine or yohimbine as chemical templates. When tested for their ability to enhance the cytotoxic activity of natural product antitumor drugs in CEM/VLB100 cells, five compounds that retained the pendant benzoyl function in an appropriate spatial orientation all modulated MDR. By contrast, compounds lacking this moiety failed to do so. These active modulators competed strongly with the 125I-labeled vinblastine analog for binding to P-glycoprotein in plasma membrane vesicles prepared from these cells. Conformational analysis using molecular mechanics revealed the structural similarities of the active modulators. Our results support the hypothesis that the relative disposition of aromatic rings and basic nitrogen atom is important for modulators of P-glycoprotein-associated MDR, and they suggest a ligand-receptor relationship for these agents. These results also provide direction for the definition of an MDR "pharmacophore."
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-2434476, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-2888464, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-2888532, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-2892943, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-2893251, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-2898941, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-2899442, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-3034908, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-3162758, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-3455678, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-3459160, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-3553950, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-3700389, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-571759, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-6089511, http://linkedlifedata.com/resource/pubmed/commentcorrection/2567994-6354435
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5128-32
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Essential features of the P-glycoprotein pharmacophore as defined by a series of reserpine analogs that modulate multidrug resistance.
pubmed:affiliation
Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN 46285.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't