Linked Life Data

2562935

Source:http://linkedlifedata.com/resource/pubmed/id/2562935

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-2-21
pubmed:abstractText
The purpose of this study was to use affinity-purified polyclonal antibodies produced against a synthetic peptide corresponding to the joining (J) region of a human T cell receptor beta chain to characterize antigen receptor expression on subpopulations of human lymphocytes. The synthetic peptide used was ANYGYTFGSGTRLTVV, corresponding to the J segment of the human beta-chain gene YT35. Biochemical characterization has previously demonstrated binding of anti-J beta peptide antibodies to the alpha/beta heterodimer and to certain immunoglobulin light chains. Flow cytometric analysis of normal human peripheral blood lymphocytes performed here, using affinity-purified antibodies to the J beta peptide, showed expression of the epitope on 50-60% of CD20 (B1)-positive B lymphocytes, and on 40-50% of CD8-positive T lymphocytes. Only background levels were observed on CD4-positive T cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
526-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Flow cytometric analysis of human lymphocytes using affinity-purified antibody to T cell receptor beta synthetic J region peptide.
pubmed:affiliation
Department of Urology, Loyola Stritch School of Medicine, Maywood, Illinois 60153.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't