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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1991-4-22
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pubmed:abstractText |
We isolated two novel mammalian kinase genes by weak cross-hybridization with v-ros oncogene. (1) A cDNA of about 7.7 kb obtained from a human placenta cDNA library carried a 4.2 kb open reading frame, and the predicted amino acid sequence of 1338 residues contained extracellular, transmembrane and tyrosine kinase domains. The overall structure including cysteine motifs in its extracellular domain and a long peptide insertion in its tyrosine kinase domain indicates that this new gene is closely related to the fms family. Consequently, the gene was designated as flt(fms-like tyrosine kinase) gene. The expression of the flt gene was examined in normal and transformed cells, and it was mapped to human chromosome 13q12-13. (2) Another new gene was found to be expressed almost exclusively in testis. The cDNA sequence analysis revealed that the predicted product carried protein kinase consensus motifs in its amino-terminal region. Comparison of the deduced amino acid sequence of this gene in the kinase domain with those of other protein kinase genes will be discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/v-ros protein, Avian sarcoma virus...
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pubmed:status |
MEDLINE
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pubmed:author |
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-10
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2562176-Amino Acid Sequence,
pubmed-meshheading:2562176-Animals,
pubmed-meshheading:2562176-Base Sequence,
pubmed-meshheading:2562176-Chromosomes, Human, Pair 13,
pubmed-meshheading:2562176-DNA,
pubmed-meshheading:2562176-DNA Probes,
pubmed-meshheading:2562176-Genes,
pubmed-meshheading:2562176-Humans,
pubmed-meshheading:2562176-Male,
pubmed-meshheading:2562176-Molecular Sequence Data,
pubmed-meshheading:2562176-Molecular Structure,
pubmed-meshheading:2562176-Multigene Family,
pubmed-meshheading:2562176-Oncogene Proteins, Viral,
pubmed-meshheading:2562176-Oncogenes,
pubmed-meshheading:2562176-Open Reading Frames,
pubmed-meshheading:2562176-Protein-Tyrosine Kinases,
pubmed-meshheading:2562176-Proto-Oncogene Proteins,
pubmed-meshheading:2562176-Rats,
pubmed-meshheading:2562176-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:2562176-Receptors, Cell Surface,
pubmed-meshheading:2562176-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2562176-Testis,
pubmed-meshheading:2562176-Vascular Endothelial Growth Factor Receptor-1
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pubmed:year |
1989
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pubmed:articleTitle |
Isolation and characterization of new mammalian kinase genes by cross hybridization with a tyrosine kinase probe.
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pubmed:affiliation |
Department of Genetics, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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