Linked Life Data

2560936

Source:http://linkedlifedata.com/resource/pubmed/id/2560936

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-5-1
pubmed:abstractText
The antibody response of eight sheep immunised against leukotriene B4-keyhold limpet haemocyanin (LTB4-KLH) was investigated. Four Suffolk x Mule sheep and two Soay sheep received subcutaneous injections of conjugate over a period of 11-21 weeks, and two Suffolk x Mule sheep received intramuscular and subcutaneous injections for 11 weeks. All eight animals reacted successfully to immunisation and produced antibodies of high affinity and specificity for LTB4. The antiplasma could be used for radioimmunoassay at dilutions of 1/10(5)-1/10(6) and the highest titres were obtained in Soay sheep. Intramuscular immunisation produced a faster but less sustained response than subcutaneous injections. The uniformity of response between animals in relation to the immunisation regime and the physico-chemical similarity of the antibodies suggest that the quality of the conjugate is the most important factor for successful antibody production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0934-9820
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Preparation of leukotriene B4 antibodies in sheep.
pubmed:affiliation
University of Oxford, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't