Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1990-3-2
|
| pubmed:abstractText |
A cultured glioma cell line, SR-B10.A, which was derived from a brain tumor induced in an adult female B10.A mouse by Rous sarcoma virus (RSV), has been established. The morphological appearance of the tumor produced by s.c. inoculating SR-B10.A cells was analogous to an astrocytoma of human glioma. Glial fibrillary acidic protein as well as S-100 protein was positive in these SR-B10.A tumor cells. A population doubling time of the cultured cells was 18.5 hours. Chromosomal analysis revealed a defect in one of the sex chromosomes. Integration of RSV genome was proven to be positive in SR-B10.A cells. It was possible to generate cytotoxic effector cells in the syngeneic B10.A mouse against SR-B10.A. The tumor-bearing syngeneic hosts harbored a suppressor activity in the splenocytes. Although recombinant human tumor necrosis factor (rH-TNF) had no growth inhibitory effect on the SR-B10.A cells in vitro, the s.c. implanted and growing tumor regressed when rH-TNF was administered intratumorally several times. In addition, this anti-tumor effect was completely abrogated when the host mice were treated with wholebody x-ray irradiation prior to the tumor cells inoculation. In contrast, neither rH-TNF (i.v.) nor cyclophosphamide (i.p.) induced the regression of SR-B10.A, indicating that efficacy of the locally administered rH-TNF is dependent on the host immune mechanism. These results suggest that SR-B10.A is a potentially useful tumor model in evaluating efficacy of immunomodulators.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0021-5031
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
59
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
173-80
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2559217-Animals,
pubmed-meshheading:2559217-Avian Sarcoma Viruses,
pubmed-meshheading:2559217-Brain Neoplasms,
pubmed-meshheading:2559217-Cell Transformation, Neoplastic,
pubmed-meshheading:2559217-Cell Transformation, Viral,
pubmed-meshheading:2559217-Chick Embryo,
pubmed-meshheading:2559217-Chromosome Aberrations,
pubmed-meshheading:2559217-Cyclophosphamide,
pubmed-meshheading:2559217-Cytotoxicity, Immunologic,
pubmed-meshheading:2559217-Disease Models, Animal,
pubmed-meshheading:2559217-Glioma,
pubmed-meshheading:2559217-Immunotherapy,
pubmed-meshheading:2559217-Mice,
pubmed-meshheading:2559217-Mice, Inbred C57BL,
pubmed-meshheading:2559217-Neoplasm Transplantation,
pubmed-meshheading:2559217-Recombinant Proteins,
pubmed-meshheading:2559217-Spleen,
pubmed-meshheading:2559217-Tumor Cells, Cultured,
pubmed-meshheading:2559217-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Potential usefulness of a cultured glioma cell line induced by Rous sarcoma virus in B10.A mouse as an immunotherapy model.
|
| pubmed:affiliation |
Department of Surgery, Gunma University School of Medicine, Maebashi, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|