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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-2-13
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pubmed:abstractText |
We screened a human thyroid cDNA library with two synthetic oligonucleotides based on the reported amino acid sequence of the 3rd and 4th transmembrane domains of a putative human TSH receptor and related receptors. The nucleotide sequence of a 4 kb clone revealed an open reading frame of 764 amino acids (86,816 Daltons) with a putative signal peptide, seven transmembrane domains, five potential glycosylation sites, and a very short intracytoplasmic region. Homology with the extracellular domain of the pig LH/CG receptor was only 33%. Chinese hamster ovary cells stably transfected with this cDNA in an expression vector generated a functional receptor, able to activate adenylate cyclase, specifically in response to TSH stimulation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1184-90
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2558651-Amino Acid Sequence,
pubmed-meshheading:2558651-Animals,
pubmed-meshheading:2558651-Base Sequence,
pubmed-meshheading:2558651-Cell Line,
pubmed-meshheading:2558651-Cloning, Molecular,
pubmed-meshheading:2558651-Codon,
pubmed-meshheading:2558651-Cricetinae,
pubmed-meshheading:2558651-Cyclic AMP,
pubmed-meshheading:2558651-DNA,
pubmed-meshheading:2558651-Gene Expression,
pubmed-meshheading:2558651-Glycosylation,
pubmed-meshheading:2558651-Humans,
pubmed-meshheading:2558651-Molecular Sequence Data,
pubmed-meshheading:2558651-Molecular Weight,
pubmed-meshheading:2558651-Receptors, LH,
pubmed-meshheading:2558651-Receptors, Thyrotropin,
pubmed-meshheading:2558651-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2558651-Transfection
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pubmed:year |
1989
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pubmed:articleTitle |
Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor.
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pubmed:affiliation |
Thyroid Molecular Biology Laboratory, Veterans' Administration Medical Center, San Francisco, California 94121.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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