Linked Life Data

2558023

Source:http://linkedlifedata.com/resource/pubmed/id/2558023

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1990-2-22
pubmed:abstractText
The genetic structure and expression of the T cell receptor (TcR) loci were examined in pre-B and early hemopoietic cells. Thirty-eight percent of Abelson murine leukemia virus-transformed pre-B cell lines were rearranged at TcR gamma. Moreover, many pre-B cell lines were rearranged at two distinct gamma loci, V gamma 1.2 and V gamma 2. The gamma rearrangements in the pre-B cell lines were similar to those observed previously in T cell lines. V + C-containing gamma mRNA was detected in two pre-B cell lines. In all other pre-B and interleukin (IL) 3-dependent lymphoid and myeloid lines examined, smaller C gamma-containing mRNA were detected. These C gamma transcripts were independent of the genetic configuration of the gamma locus. In contrast, the TcR alpha and beta loci were in the germ-line configuration in all non-T cell lines examined and mRNA encoding these loci were not detected. When IL3-dependent lymphoid and myeloid cell lines were transformed to growth factor independence by a non-autocrine mechanism, no mRNA transcripts encoding TcR C gamma were detected. However, TcR C gamma mRNA transcripts were detected in factor-independent cell lines that arose by an autocrine mechanism. The cell cycle expression of C gamma was compared with protooncogenes and other marker genes previously shown to be cell cycle specific. mRNA transcripts encoding C gamma were detected in the highest amounts 4-8 h after IL 3, but not phorbol myristate acetate, addition. A similar time period of expression was observed with ornithine decarboxylase which has been shown to be expressed in G1 phase. These observations indicate that TcR gamma is often rearranged in pre-B cell lines and may be directly regulated by IL3 in IL3-dependent cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2303-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Structure and expression of the T cell receptor gamma locus in pre-B and early hemopoietic cells.
pubmed:affiliation
Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't