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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6 Pt 1
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pubmed:dateCreated |
1990-1-24
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pubmed:abstractText |
Addition of EGF to human epidermoid carcinoma A431 cells increases the rate of fluid-phase pinocytosis 6-10-fold as measured by horseradish peroxidase uptake (Haigler, H.T., J. A. McKanna, and S. Cohen. 1979. J. Cell Biol. 83:82-90). We show here that in the absence of extracellular Na+ or in the presence of amiloride the stimulation of pinocytosis by EGF is substantially reduced. Amiloride had no effect on the endocytosis of EGF itself or of transferrin, demonstrating that the receptor-mediated endocytotic pathway operated normally under conditions that blocked stimulated pinocytosis. Amiloride blocked EGF-stimulated pinocytosis in both HCO3(-)-containing and HCO3(-)-free media. The EGF-stimulated pinocytotic activity can frequently be localized to areas of the cell where membrane spreading and ruffling are taking place. These results demonstrate that (a) EGF induces a distinct amiloride-sensitive endocytotic pathway on A431 cells; (b) occupied EGF receptors do not utilize this pathway for their own entry; (c) endocytosis of occupied EGF receptors is not in itself sufficient to stimulate pinocytosis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-1001364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-13597891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-14293043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-1460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-2521920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-2538338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-2867759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-2881559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-2887575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-2918022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-2921284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3011419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3011821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3018928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3023396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3090687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-313931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-315943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-315944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3262110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3428267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3498714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-3918045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-4347251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6091747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6095096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6138252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6141171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6195155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6300103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6309862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6323465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-637870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6410286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6979542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-6996834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-7070509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-7463480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556406-82969
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
109
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2731-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2556406-Amiloride,
pubmed-meshheading:2556406-Bicarbonates,
pubmed-meshheading:2556406-Carrier Proteins,
pubmed-meshheading:2556406-Cell Line,
pubmed-meshheading:2556406-Endocytosis,
pubmed-meshheading:2556406-Epidermal Growth Factor,
pubmed-meshheading:2556406-Humans,
pubmed-meshheading:2556406-Kinetics,
pubmed-meshheading:2556406-Pinocytosis,
pubmed-meshheading:2556406-Sodium-Hydrogen Antiporter,
pubmed-meshheading:2556406-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Distinct endocytotic pathways in epidermal growth factor-stimulated human carcinoma A431 cells.
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pubmed:affiliation |
Department of Biochemistry, University of Dundee, UK.
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pubmed:publicationType |
Journal Article
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