|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
35
|
|
pubmed:dateCreated |
1990-1-19
|
|
pubmed:abstractText |
Transfection of the cDNA encoding the activated c-raf-1 protein or addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) or dibutyryl cAMP to NIH/3T3 cells activated the c-fos gene enhancer linked to the chloramphenicol acetyltransferase or luciferase reporter gene. Prolonged treatment of NIH/3T3 cells with phorbol 12,13-dibutyrate caused down-regulation of protein kinase C. In these cells, addition of TPA did not stimulate the c-fos gene enhancer any more, but transfection of the c-raf-1 cDNA or addition of dibutyryl cAMP still stimulated the c-fos gene enhancer to the same extent as those induced in the control cells. Transfection of the c-raf-1 cDNA or addition of TPA to NIH/3T3 cells stimulated the serum response element and TPA response element but not the cAMP response element. In contrast, addition of dibutyryl cAMP to NIH/3T3 cells stimulated the cAMP response element but not the serum response element or TPA response element. These results indicate that the activated c-raf-1 protein stimulates the serum response element and TPA response element in a manner independent of protein kinase C and cAMP-dependent protein kinase. Since the c-fos gene enhancer has been shown to contain the serum response element and cAMP response element, it is most likely that the c-raf-1 protein is involved in the regulation of c-fos gene expression through the serum response element.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
264
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
20855-8
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:2556385-Animals,
pubmed-meshheading:2556385-Base Sequence,
pubmed-meshheading:2556385-Bucladesine,
pubmed-meshheading:2556385-Cells, Cultured,
pubmed-meshheading:2556385-Culture Media,
pubmed-meshheading:2556385-Enhancer Elements, Genetic,
pubmed-meshheading:2556385-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:2556385-Mice,
pubmed-meshheading:2556385-Molecular Sequence Data,
pubmed-meshheading:2556385-Nuclear Proteins,
pubmed-meshheading:2556385-Protein Kinase C,
pubmed-meshheading:2556385-Protein-Tyrosine Kinases,
pubmed-meshheading:2556385-Proto-Oncogene Proteins,
pubmed-meshheading:2556385-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:2556385-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:2556385-Proto-Oncogenes,
pubmed-meshheading:2556385-Serum Response Factor,
pubmed-meshheading:2556385-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2556385-Transfection
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Activation of the serum response element and 12-O-tetradecanoylphorbol-13-acetate response element by the activated c-raf-1 protein in a manner independent of protein kinase C.
|
|
pubmed:affiliation |
Department of Biochemistry, Kobe University School of Medicine, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
