Linked Life Data

2556360

Source:http://linkedlifedata.com/resource/pubmed/id/2556360

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1990-1-25
pubmed:abstractText
The 5-AMPase activity of the ectoenzyme 5-nucleotidase has been measured in a variety of cell lines, using intact cells. Human cell types showed two orders of magnitude higher enzyme activity than mouse cell lines. The ectoenzyme is inhibited by adenosine 5-(alpha, beta-methylene) diphosphate and Concanavalin A. A different extent of 5-nucleotidase lectin inhibition was observed in the studied cell lines, suggesting that the corresponding ectoenzymes are glycoproteins with a different type or degree, or both, of glycosylation. The 5-nucleotidase activity increased during subculture and decreased after cell transformation. Generally, the 5-nucleotidase activity was two- to five-fold higher in monolayer than in suspension cell culture. A relation between cell growth and 5-AMPase activity was also observed. Enzyme activity increased at the end of the lag phase (glioblastoma cells) or during the exponential phase (the other two cell lines). After confluence, the activity decreased to the initial or even lower range of activity. Observed activity variations with cell proliferation correlate with modifications of 5-AMPase activity during subculture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0883-8364
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1055-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
5-nucleotidase activity in cultured cell lines. Effect of different assay conditions and correlation with cell proliferation.
pubmed:affiliation
Departamento de Bioquímica, Max-Planck Institut für Biochemie, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't