|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0006100,
umls-concept:C0020291,
umls-concept:C0031586,
umls-concept:C0031621,
umls-concept:C0031670,
umls-concept:C0033567,
umls-concept:C0087111,
umls-concept:C0220781,
umls-concept:C0225336,
umls-concept:C0443252,
umls-concept:C1879547,
umls-concept:C1883254,
umls-concept:C1948023
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1990-1-11
|
|
pubmed:abstractText |
Bovine pulmonary artery endothelial cells (BPAEC) were prelabeled with [3H]choline or [3H]myristic acid to selectively label endogenous phosphatidylcholine. BPAEC were stimulated with ATP and bradykinin (BK), and phospholipase D (PLD) activation was detected as a 4-fold increase in [3H]choline in cells prelabeled with [3H]choline or as a 2- to 3-fold increase in [3H]phosphatidylethanol in cells prelabeled with [3H]myristic acid and stimulated in the presence of ethanol. Pretreatment of BPAEC with 0.1 microM phorbol 12-myristate 13-acetate (PMA) for 22 hr completely inhibited agonist-induced PLD activation, whereas prostacyclin synthesis and [3H]phosphoinositide ([3H]PIns) hydrolysis were enhanced in pretreated cells. Long-term PMA treatment thus dissociates agonist-induced PLD activation from [3H]PIns hydrolysis, and agonist-induced prostacyclin synthesis is not dependent upon PLD activation.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/6-Ketoprostaglandin F1 alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Bradykinin,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Myristic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Myristic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
30
|
|
pubmed:volume |
165
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
319-26
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:2556140-6-Ketoprostaglandin F1 alpha,
pubmed-meshheading:2556140-Animals,
pubmed-meshheading:2556140-Bradykinin,
pubmed-meshheading:2556140-Cattle,
pubmed-meshheading:2556140-Cells, Cultured,
pubmed-meshheading:2556140-Endothelium, Vascular,
pubmed-meshheading:2556140-Enzyme Activation,
pubmed-meshheading:2556140-Hydrolysis,
pubmed-meshheading:2556140-Inositol,
pubmed-meshheading:2556140-Inositol Phosphates,
pubmed-meshheading:2556140-Kinetics,
pubmed-meshheading:2556140-Myristic Acid,
pubmed-meshheading:2556140-Myristic Acids,
pubmed-meshheading:2556140-Phorbol Esters,
pubmed-meshheading:2556140-Phosphatidylinositols,
pubmed-meshheading:2556140-Phospholipase D,
pubmed-meshheading:2556140-Phospholipases,
pubmed-meshheading:2556140-Pulmonary Artery,
pubmed-meshheading:2556140-Tetradecanoylphorbol Acetate
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Long-term phorbol ester treatment dissociates phospholipase D activation from phosphoinositide hydrolysis and prostacyclin synthesis in endothelial cells stimulated with bradykinin.
|
|
pubmed:affiliation |
Department of Pathology, St. Louis University School of Medicine, Missouri 63104.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
