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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1989-12-28
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pubmed:abstractText |
Two azole resistant isolates of Saccharomyces cerevisiae carried mutations allelic to erg 3 and were blocked to differing degrees at the C5-6 desaturation step of ergosterol biosynthesis. When treated with the sterol 14 alpha-demethylation inhibitor fluconazole the wild-type sensitive strain accumulated lanosterol and 14 alpha-methyl-erogosta-8,24(28)-dien-3 beta, 6 alpha-diol (14-methyl-3,6 diol). The stringent desaturase mutant, A2, accumulated 14 alpha-methyl-8,24(28)-dien-3 beta-ol (14-methyl fecosterol) and lanosterol as the major sterol components when treated with fluconazole. Resistant isolate A3 accumulated 14-methyl-3,6-diol, 14-methyl fecosterol, and lanosterol and was only partially blocked at sterol C5-6 desaturation. We conclude that functional sterol C5-6 desaturase is required for the synthesis of 14-methyl-3,6-diol under conditions of azole inhibition. We present a new hypothesis for the mode of action of azole antifungals based on the inability of 14-methyl-3,6-diol to support growth, and suggest that growth can occur through utilisation of 14-methyl fecosterol, produced by a combination of azole inhibition and defective sterol C5-6 desaturation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antifungal Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Azoles,
http://linkedlifedata.com/resource/pubmed/chemical/Fluconazole,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Sterols,
http://linkedlifedata.com/resource/pubmed/chemical/sterol delta-5 desaturase
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1170-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2556119-Antifungal Agents,
pubmed-meshheading:2556119-Azoles,
pubmed-meshheading:2556119-Drug Resistance, Microbial,
pubmed-meshheading:2556119-Fluconazole,
pubmed-meshheading:2556119-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:2556119-Mutation,
pubmed-meshheading:2556119-Oxidoreductases,
pubmed-meshheading:2556119-Saccharomyces cerevisiae,
pubmed-meshheading:2556119-Sterols
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pubmed:year |
1989
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pubmed:articleTitle |
Defective sterol C5-6 desaturation and azole resistance: a new hypothesis for the mode of action of azole antifungals.
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pubmed:affiliation |
University Department of Pharmacology and Therapeutics, Sheffield University, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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