Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1989-12-29
pubmed:abstractText
Expression of the L1 region of adenovirus is temporally regulated by alternative splicing to yield two major RNAs encoding the 52- to 55-kilodalton (52-55K) and IIIa polypeptides. The distal acceptor site (IIIa) is utilized only during the late phase of infection, whereas the proximal site (52-55K) is used at both early and late times. Several parameters that might affect this alternative splicing were tested by using expression vectors carrying the L1 region or mutated versions of it. In the absence of a virus-encoded or -induced factor(s), only the 52-55K acceptor was used. Decreasing the distance between the donor and the IIIa acceptor had no effect. Removal of the 52-55K acceptor induced IIIa splicing slightly, implying competition between the two acceptors. Fusion of the IIIa exon to the 52-55K intron greatly enhanced splicing of the IIIa junction, suggesting that the IIIa exon does not contain sequences that inhibit splicing. Thus, the lack of splicing to the IIIa acceptor in the absence of a virus-encoded or -induced factor(s) is probably due to the absence of a favorable sequence and/or the presence of a negative element 5' of the IIIa splice junction, or both. The presence of several adenovirus gene products, including VA RNAs, the E2A DNA-binding protein, and the products of E1A and E1B genes, did not facilitate use of the IIIa acceptor. In contrast, the simian virus 40 early proteins, probably large T antigen, induced IIIa splicing. This result, together with those of earlier studies, suggest that T antigen plays a role in modulation of alternative RNA splicing.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-114304, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-226719, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2415717, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2427200, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2431291, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2432392, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2493994, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2835666, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2840286, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2850262, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2851720, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2879637, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2880558, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2903048, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2943217, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2945931, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2983309, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-2994004, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3017190, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3029566, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3215513, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3238923, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3304142, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3323803, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3658675, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-3925161, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-4057354, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-537065, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6091052, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6099327, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6204770, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6207932, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6252477, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6254000, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6260373, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6330731, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6432335, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-6688459, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-688389, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-7063411, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-7207591, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-7254339, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-7411632, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-7460018, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555691-886304
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4364-71
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
cis-acting elements and a trans-acting factor affecting alternative splicing of adenovirus L1 transcripts.
pubmed:affiliation
Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't