Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1989-12-1
pubmed:abstractText
Previous studies have indicated that the ATP-dependent 26S protease complex that degrades proteins conjugated to ubiquitin is formed by the assembly of three factors in an ATP-requiring process. We now identify one of the factors as the 20S "multicatalytic" protease, a complex of low molecular weight subunits widely distributed in eukaryotic cells. Comparison of the subunit compositions of purified 20S and 26S complexes indicates that the former is an integral part of the latter. By the use of detergent treatment to activate latent protease activity, we show that the 20S protease becomes incorporated into the 26S complex in the ATP-dependent assembly process. It thus seems that the 20S protease is the "catalytic core" of the 26S complex of the ubiquitin proteolytic pathway.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2423694, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2536933, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2538923, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2539595, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2825735, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2833171, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2842333, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2844803, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-2988526, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3003114, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3025922, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3029081, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3042462, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3095325, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3123994, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3141402, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3277060, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3298229, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3524688, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3530330, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-35530, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3611103, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3637121, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3890839, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3890840, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-3896852, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-468813, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-6287917, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-6305978, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-6324208, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-6338156, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-6364139, http://linkedlifedata.com/resource/pubmed/commentcorrection/2554287-6769112
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7751-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
ATP-dependent incorporation of 20S protease into the 26S complex that degrades proteins conjugated to ubiquitin.
pubmed:affiliation
Unit of Biochemistry, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't