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pubmed-article:2554240pubmed:abstractTextWe have optimized the conditions for efficient NIH3T3 focus formation by calcium phosphate transfection of proviral Abelson-murine leukemia virus (A-MuLV) plasmid DNA. Linearized pA-MuLV, P120 or P160 strains, when transfected with calf thymus carrier DNA, will produce 40-50 foci/100 ng pA-MuLV without co-transfecting Moloney-murine leukemia virus (Mo-MuLV) plasmid DNA.lld:pubmed
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pubmed-article:2554240pubmed:articleTitleTransformation of NIH3T3 cells by A-MuLV proviral DNA: effect of plasmid linearization and carrier DNA on transformation efficiency.lld:pubmed
pubmed-article:2554240pubmed:affiliationWistar Institute, Philadelphia, Pennsylvania 19104.lld:pubmed
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