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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1989-12-21
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pubmed:abstractText |
We have optimized the conditions for efficient NIH3T3 focus formation by calcium phosphate transfection of proviral Abelson-murine leukemia virus (A-MuLV) plasmid DNA. Linearized pA-MuLV, P120 or P160 strains, when transfected with calf thymus carrier DNA, will produce 40-50 foci/100 ng pA-MuLV without co-transfecting Moloney-murine leukemia virus (Mo-MuLV) plasmid DNA.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1411-3
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2554240-Abelson murine leukemia virus,
pubmed-meshheading:2554240-Animals,
pubmed-meshheading:2554240-Cell Transformation, Neoplastic,
pubmed-meshheading:2554240-Cells, Cultured,
pubmed-meshheading:2554240-DNA,
pubmed-meshheading:2554240-DNA, Viral,
pubmed-meshheading:2554240-Leukemia Virus, Murine,
pubmed-meshheading:2554240-Mice,
pubmed-meshheading:2554240-Mice, Inbred Strains,
pubmed-meshheading:2554240-Plasmids,
pubmed-meshheading:2554240-Proviruses,
pubmed-meshheading:2554240-Salmon,
pubmed-meshheading:2554240-Transfection
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pubmed:year |
1989
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pubmed:articleTitle |
Transformation of NIH3T3 cells by A-MuLV proviral DNA: effect of plasmid linearization and carrier DNA on transformation efficiency.
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pubmed:affiliation |
Wistar Institute, Philadelphia, Pennsylvania 19104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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