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rdf:type |
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lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0003261,
umls-concept:C0012472,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0021759,
umls-concept:C0021760,
umls-concept:C0178719,
umls-concept:C0441889,
umls-concept:C0442805,
umls-concept:C1155003,
umls-concept:C1280500
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pubmed:issue |
2
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pubmed:dateCreated |
1989-12-13
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pubmed:abstractText |
Effects of prostaglandin E2 (PGE2) on murine B-cell activation induced by two distinct B-cell differentiation factors, B151-TRF1/IL-5 and B151-TRF2, were examined. A final differentiation of unprimed B cells into IgM-producing cells induced by B151-TRF2 was markedly inhibited by PGE2 at physiological concentrations (around 10(-8) M), whereas B151-TRF1/IL-5- induced antibody responses of unprimed as well as activated B cells were not affected by PGE2, even at 10(-6) M. B-cell responses induced by B151-TRF2-like factors from autoimmune-prone MRL/lpr mice were also inhibited by PGE2. Biphasic increases in intracellular cyclic AMP (cAMP) levels were induced by culturing B cells with 10(-6) or 10(-8) M PGE2: rapid increases within 8 min and delayed increases around 16 hr. The direct addition of dibutyryl cAMP to cultures of B cells resulted in marked inhibition of antibody responses when stimulated with B151-TRF2 but not with B151-TRF1/IL-5. The B151-TRF2-induced antibody responses were also inhibited by cAMP-elevating reagents such as forskolin, cholera toxin and theophylline. Furthermore, 2'.5'-dideoxyadenosine, which is an inhibitor of adenylate cyclase, prevented the PGE2-mediated cAMP accumulation in unprimed B cells as well as the PGE2-mediated inhibition of B151-TRF2-induced B-cell responses when added at the initiation of culture. These results suggest that PGE2 inhibits B151-TRF2-induced antibody responses through the activation of adenylate cyclase and subsequent accumulation of intracellular cAMP, whereas B151-TRF1/IL-5-responsive B cells are resistant to the inhibitory effect of PGE2 and cAMP.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-144506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-208069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-227981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2408764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2458941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2487675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-271970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2823259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2828473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2857750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2939856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-2989362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3027169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3037375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3090140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3155734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3263941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3316381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3489143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3489778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3491148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3509923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3519774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3543118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-3872889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-4343159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-593374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-6086754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-6267587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-6287286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-6332851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-6600490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-6978818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-6987016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2553585-7028332
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2',5'-dideoxyadenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dideoxyadenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
154-62
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2553585-Adjuvants, Immunologic,
pubmed-meshheading:2553585-Animals,
pubmed-meshheading:2553585-B-Lymphocytes,
pubmed-meshheading:2553585-Bucladesine,
pubmed-meshheading:2553585-Cells, Cultured,
pubmed-meshheading:2553585-Cyclic AMP,
pubmed-meshheading:2553585-Dideoxyadenosine,
pubmed-meshheading:2553585-Dinoprostone,
pubmed-meshheading:2553585-Immunoglobulin M,
pubmed-meshheading:2553585-Interleukin-4,
pubmed-meshheading:2553585-Interleukin-5,
pubmed-meshheading:2553585-Lymphocyte Activation,
pubmed-meshheading:2553585-Mice
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pubmed:year |
1989
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pubmed:articleTitle |
Different effect of prostaglandin E2 on B-cell activation by two distinct B-cell differentiation factors, B151-TRF1/IL-5 and B151-TRF2: selective inhibition of B151-TRF2-induced antibody response through increases in intracellular cyclic AMP levels.
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pubmed:affiliation |
Division of Oncogenesis, Osaka University Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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