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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-11-13
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pubmed:abstractText |
Treatment of Jurkat T-cells with anti-CD-3 monoclonal antibodies resulted in the rapid and transient activation of a serine kinase which utilized the microtubule-associated protein, MAP-2, as a substrate in vitro. The kinase was also activated on treatment of Jurkat cells with phytohaemagglutinin, but with a different time course. The activation of the MAP-2 kinase by anti-CD-3 antibodies was dose-dependent, with maximal activity observed at concentrations of greater than 500 ng/ml. Normal human E-rosette-positive T-cells also exhibited induction of MAP-2 kinase activity during anti-CD-3 treatment. The enzyme was optimally active in the presence of 2 mM-Mn2+; lower levels of activity were observed with Mg2+, even at concentrations up to 20 mM. The kinase was partially purified by passage over DE-52 Sephacel with the activity eluting as a single peak at 0.25 M-NaCl. The molecular mass was estimated to be 45 kDa by gel filtration. The activation of the MAP-2 kinase was probably due to phosphorylation of this enzyme as treatment with alkaline phosphatase diminished its activity. These data demonstrate that the stimulation of T-cells through the CD-3 complex results in the activation of a novel serine kinase which may be critically involved in signal transduction in these cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-233982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2417941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2440631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2824482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2842341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2842685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2851989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2858484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2951373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2951732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2960679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2961214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2969888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-2992362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3106485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3113737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3161747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3287375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3511044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3548773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3584129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3683742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3871779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3920212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3926881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3934263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-3949805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-457745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6120944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6157683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6173766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6188161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6196603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6203790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6232463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6238627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6277931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6281080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6305960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6321035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6321473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6321474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-6415161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-7142990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552997-7159453
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
262
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
449-56
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2552997-Antibodies, Monoclonal,
pubmed-meshheading:2552997-Antigens, CD3,
pubmed-meshheading:2552997-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2552997-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:2552997-Cell Line,
pubmed-meshheading:2552997-Dose-Response Relationship, Immunologic,
pubmed-meshheading:2552997-Enzyme Activation,
pubmed-meshheading:2552997-Enzyme Induction,
pubmed-meshheading:2552997-Humans,
pubmed-meshheading:2552997-Membrane Glycoproteins,
pubmed-meshheading:2552997-Protein Kinases,
pubmed-meshheading:2552997-Receptors, Antigen, T-Cell,
pubmed-meshheading:2552997-T-Lymphocytes
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pubmed:year |
1989
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pubmed:articleTitle |
Complexing of the CD-3 subunit by a monoclonal antibody activates a microtubule-associated protein 2 (MAP-2) serine kinase in Jurkat cells.
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