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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1989-11-15
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pubmed:abstractText |
Genome segments 2, 6, 8, and 9 of bluetongue virus (BTV) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States BTV prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to BTV-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones failed to detect the related orbivirus, epizootic hemorrhagic disease virus.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0002-9645
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1684-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2552873-Animals,
pubmed-meshheading:2552873-Blotting, Northern,
pubmed-meshheading:2552873-Bluetongue,
pubmed-meshheading:2552873-Bluetongue virus,
pubmed-meshheading:2552873-Cloning, Molecular,
pubmed-meshheading:2552873-DNA Probes,
pubmed-meshheading:2552873-Electrophoresis, Agar Gel,
pubmed-meshheading:2552873-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2552873-Hemorrhagic Fevers, Viral,
pubmed-meshheading:2552873-Nucleic Acid Hybridization,
pubmed-meshheading:2552873-RNA, Double-Stranded,
pubmed-meshheading:2552873-RNA, Viral,
pubmed-meshheading:2552873-Reoviridae,
pubmed-meshheading:2552873-Reoviridae Infections,
pubmed-meshheading:2552873-Vero Cells
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pubmed:year |
1989
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pubmed:articleTitle |
Molecular cloning of serogroup- and serotype-specific genome segments from bluetongue virus serotype 11.
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pubmed:affiliation |
Department of Veterinary Microbiology, School of Veterinary Medicine, University of California, Davis 95616.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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