Linked Life Data

2552301

Source:http://linkedlifedata.com/resource/pubmed/id/2552301

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-11-13
pubmed:abstractText
The genetic relatedness of various human rotavirus strains was examined by RNA-RNA hybridization in which 32P-labelled single stranded RNAs produced by in vitro transcription from viral RNAs were used as probes. Denatured genomic double stranded RNAs were hybridized to the probes under highly stringent conditions and the resulting hybrids were fractionated by polyacrylamide gel electrophoresis. Based on the hybridization patterns obtained with probes made from prototype strains Wa (subgroup II, long RNA electropherotype), DS-1 (subgroup I, short RNA electropherotype) and AU-1 (subgroup I, long RNA electropherotype), we have observed that human rotaviruses fall into three distinct gene groups which we have termed 'genogroups'. Identification of genogroups among rotavirus isolates will prove to be a valuable asset for the analysis of naturally occurring reassortants, to trace interspecies transmission of animal rotaviruses to man or vice versa and to identify rotaviruses from environmental sources with regard to their original host species. Furthermore, such an approach will contribute to our understanding of the evolution of rotavirus genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0890-8508
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Identification of rotavirus genogroups by RNA-RNA hybridization.
pubmed:affiliation
Department of Laboratory Medicine, Akita University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't