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rdf:type |
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lifeskim:mentions |
umls-concept:C0003483,
umls-concept:C0007452,
umls-concept:C0020792,
umls-concept:C0025255,
umls-concept:C0086376,
umls-concept:C0205164,
umls-concept:C0249197,
umls-concept:C0288472,
umls-concept:C0682970,
umls-concept:C1267092,
umls-concept:C1420626
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pubmed:issue |
3
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pubmed:dateCreated |
1989-10-25
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pubmed:abstractText |
At least two GTP-binding proteins (G proteins) with Mr values of about 20,000 were extracted from bovine aortic smooth muscle membranes by sodium cholate. The most abundant G protein (22K G) was purified to near homogeneity by successive column chromatographies of Ultrogel AcA-44, phenyl-Sepharose CL-4B, hydroxyapatite and Mono Q HR5/5. 22K G showed kinetic and physical properties very similar to those of smg p21, a G protein recently isolated from bovine brain and human platelet membranes, having the same effector domain as ras p21s. Moreover, 22K G was recognized specifically by the anti-smg p21 antibody. These results indicate that the major G protein in bovine aortic smooth muscle membranes is smg p21.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1418-27
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2551287-Animals,
pubmed-meshheading:2551287-Aorta,
pubmed-meshheading:2551287-Cattle,
pubmed-meshheading:2551287-Cell Membrane,
pubmed-meshheading:2551287-Chromatography,
pubmed-meshheading:2551287-Chromatography, Gel,
pubmed-meshheading:2551287-Chromatography, High Pressure Liquid,
pubmed-meshheading:2551287-Chromatography, Ion Exchange,
pubmed-meshheading:2551287-Durapatite,
pubmed-meshheading:2551287-GTP-Binding Proteins,
pubmed-meshheading:2551287-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:2551287-Guanosine Triphosphate,
pubmed-meshheading:2551287-Hydroxyapatites,
pubmed-meshheading:2551287-Immunoblotting,
pubmed-meshheading:2551287-Molecular Weight,
pubmed-meshheading:2551287-Muscle, Smooth, Vascular,
pubmed-meshheading:2551287-Proto-Oncogene Proteins,
pubmed-meshheading:2551287-Proto-Oncogene Proteins p21(ras),
pubmed-meshheading:2551287-Thionucleotides
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pubmed:year |
1989
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pubmed:articleTitle |
Identification of a major GTP-binding protein in bovine aortic smooth muscle membranes as smg p21, a GTP-binding protein having the same effector domain as ras p21s.
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pubmed:affiliation |
Department of Internal Medicine, Kobe University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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