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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1989-10-24
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pubmed:abstractText |
Expression of the amiloride-sensitive sodium channel was examined in Xenopus oocytes that were microinjected with A6 cell mRNA. Amiloride-inhibitable 22Na flux could be measured in intact oocytes 2-3 days after injection with 25 ng of poly(A)+ RNA isolated from aldosterone-treated A6 cells. The rate of 22Na uptake was approximately 15-fold greater in oocytes microinjected with 25 ng of poly(A)+ RNA than in water-injected control oocytes. An increase in 22Na uptake by mRNA-injected oocytes occurred whether the mRNA was isolated from A6 cells grown on a porous or nonporous support. In the presence of 4 mM NaCl, amiloride caused dose-dependent inhibition of 22Na uptake in mRNA-injected oocytes, which was half-maximal at 6 x 10(-8) M. Both 1 microM amiloride and 0.1 microM benzamil inhibited 22Na uptake in mRNA-injected oocytes by greater than 95%, whereas less than 50% inhibition occurred with 1 microM 5-(N-ethyl-N-isopropyl)amiloride. When A6 cell mRNA was size fractionated by sucrose density-gradient centrifugation, amiloride-sensitive 22Na uptake was expressed predominantly by oocytes injected with mRNA from two contiguous fractions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-13544986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2419323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2420186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2440032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2440868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2446136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2451832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2580832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-2852254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-3002185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-3110149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-316451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-3194754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-3399891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-3473504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-4109871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-6267945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-6269434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-6325248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-6470000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2550939-6614153
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7295-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2550939-Amiloride,
pubmed-meshheading:2550939-Animals,
pubmed-meshheading:2550939-Cell Line,
pubmed-meshheading:2550939-Cells, Cultured,
pubmed-meshheading:2550939-Female,
pubmed-meshheading:2550939-Kinetics,
pubmed-meshheading:2550939-Membrane Proteins,
pubmed-meshheading:2550939-Microinjections,
pubmed-meshheading:2550939-Oocytes,
pubmed-meshheading:2550939-RNA, Messenger,
pubmed-meshheading:2550939-Sodium,
pubmed-meshheading:2550939-Sodium Channels,
pubmed-meshheading:2550939-Xenopus laevis
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pubmed:year |
1989
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pubmed:articleTitle |
Functional expression of the amiloride-sensitive sodium channel in Xenopus oocytes.
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pubmed:affiliation |
Department of Medicine, University of Pennsylvania, Philadelphia 19104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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