Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-9-8
pubmed:abstractText
Biochemical and immunochemical studies were undertaken to quantify the effects of cyclic AMP on cyclic AMP-dependent protein kinase subunit levels in nuclei of H4IIE hepatoma cells. Dibutyryl cyclic AMP (10 microM) caused a significant biphasic (10 and 120 min after stimulation) increase in total nuclear protein kinase activity. The increase observed 10 min after dibutyryl cyclic AMP stimulation was primarily due to an approx. 3-fold increase of catalytic (C) subunit activity, whereas the change observed 120 min after stimulation consisted of an increase in both C subunit and cyclic AMP-independent protein kinase activities. Analysis of nuclear protein extracts by photoaffinity labelling with 8-azido cyclic [32P]AMP identified only the type II regulatory subunit (RII), but not the type I regulatory subunit (RI). Analysis of nuclear RII variants by two-dimensional gel electrophoresis demonstrated that dibutyryl cyclic AMP caused the appearance of two RII variant forms which were not present in the nuclei of unstimulated cells. Using affinity-purified polyclonal antibodies and immunoblotting procedures, we identified an approx. 2-fold increase in the RII and C subunits in nuclear extracts of dibutyryl cyclic AMP-treated hepatoma cells. Finally, the RI, RII and C subunits were quantified by an e.l.i.s.a. which indicated that dibutyryl cyclic AMP increased nuclear RII and C subunits levels biphasically, reaching peak values 10 and 120 min after the initial stimulation. Nuclear RI subunit levels were not affected. These results provide qualitative as well as quantitative evidence for a modulation by cyclic AMP of the nuclear RII and C subunit levels in rat H4IIE hepatoma cells, and indicate a relatively rapid but temporarily limited dibutyryl cyclic AMP-induced translocation of the RII and C subunits to nuclear sites.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-13293190, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-165513, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-16921, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-180001, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-207579, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-218941, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-222123, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-224463, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2411419, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2579989, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2821622, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2837663, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2842590, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2885756, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2981071, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2988780, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2990047, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2993318, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2998755, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-2999799, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-3005039, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-3139655, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-42014, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-4310608, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-4375767, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6094570, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6096026, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6096365, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6131900, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6160145, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6196356, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6244301, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6255017, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6260518, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6267050, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6269181, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6272695, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6277249, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6291025, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6292198, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6300127, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6353247, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-6760854, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548485-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
673-82
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Modulation of nuclear cyclic AMP-dependent protein kinase in dibutyryl cyclic AMP-treated rat H4IIE hepatoma cells.
pubmed:affiliation
Department of Molecular Biology, Northwestern University Medical School, Chicago, IL 60611.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't