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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1989-9-19
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pubmed:abstractText |
Incubation of topologically relaxed plasmid DNA with simian virus 40 (SV40) large tumor antigen (T antigen), ATP, and eubacterial DNA topoisomerase I resulted in the formation of highly positively supercoiled DNA. Eukaryotic DNA topoisomerase I could not substitute for eubacterial DNA topoisomerase 1 in this reaction. Furthermore, the addition of eukaryotic topoisomerase I to a preincubated reaction mixture containing both T antigen and eubacterial topoisomerase I caused rapid relaxation of the positively supercoiled DNA. These results suggest that SV40 T antigen can introduce topoisomerase-relaxable supercoils into DNA in a reaction coupled to ATP hydrolysis. We interpret the observed T antigen supercoiling reaction in terms of a recently proposed twin-supercoiled-domain model that describes the mechanics of DNA helix-tracking processes. According to this model positive and negative supercoils are generated ahead of and behind the moving SV40 T antigen, respectively. The preferential relaxation of negative supercoils by eubacterial DNA topoisomerase I explains the accumulation of positive supercoils in the DNA template. The supercoiling assay using DNA conformation-specific eubacterial DNA topoisomerase I may be of general use for the detection of ATP-dependent DNA helix-tracking proteins.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-153201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2535966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2536723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-276855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2825183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2826443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2826446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2835168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2847873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-2983081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-3018548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-3019672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-3020552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-3027371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-3035543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-3281259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6095264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6248234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6256081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6265100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6267594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6288959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6298451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6298452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548199-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6121-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2548199-Adenosine Triphosphate,
pubmed-meshheading:2548199-Antigens, Polyomavirus Transforming,
pubmed-meshheading:2548199-Cell Line,
pubmed-meshheading:2548199-DNA, Superhelical,
pubmed-meshheading:2548199-DNA Topoisomerases, Type I,
pubmed-meshheading:2548199-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:2548199-Escherichia coli,
pubmed-meshheading:2548199-Humans,
pubmed-meshheading:2548199-Nucleic Acid Conformation,
pubmed-meshheading:2548199-Plasmids,
pubmed-meshheading:2548199-Polyethylene Glycols,
pubmed-meshheading:2548199-Simian virus 40,
pubmed-meshheading:2548199-Templates, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
Template supercoiling during ATP-dependent DNA helix tracking: studies with simian virus 40 large tumor antigen.
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pubmed:affiliation |
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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