Linked Life Data

2548188

Source:http://linkedlifedata.com/resource/pubmed/id/2548188

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1989-9-12
pubmed:abstractText
The addition of the platelet-activating factor (PAF) to neutrophils causes an increase in cytoskeletal actin, a rise in the intracellular concentration of free calcium, release of arachidonic acid, and the synthesis of PAF. The PAF synthesis in human neutrophils stimulated by PAF is greatly potentiated by the human granulocyte-macrophage colony-stimulating factor. Incubation of human neutrophils with the tumor copromoter phorbol 12-myristate 13-acetate (PMA) for 3 min prior to the addition of the stimulus inhibits all these responses produced by PAF. The inhibition is prevented when the cells are incubated with protein kinase C inhibitors such as 1-(5-isoquinolinesulfonyl)-2-methylpiperazine for 5 min prior to the addition of PMA. The rise in the intracellular concentration of free calcium in human neutrophils stimulated with leukotriene B4 is also inhibited by PMA, and this inhibition is prevented by protein kinase C inhibitors such as staurosporine. Unlike PMA, the inactive ester 4 alpha-phorbol 12,13-didecanoate has no inhibitory effect on the stimulated rise in the intracellular concentration of free calcium. The binding of either PAF or leukotriene B4 to intact cells is inhibited by PMA. The most important finding of the present studies is that PMA interferes with the binding of PAF and leukotriene B4 to their respective receptors. Whether PMA inhibits the binding of these lipid mediators by activating protein kinase C or by perturbing the membrane directly remains to be elucidated.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-13671378, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-2494986, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3014651, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3016027, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3019772, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3021215, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3022313, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3041911, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3082894, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3083776, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3092807, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3128281, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3140244, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3226310, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3304132, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3348814, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3545319, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-3932407, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-4427075, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-5721037, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-6088613, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-6091126, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-7207461, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-7386258, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-7430120, http://linkedlifedata.com/resource/pubmed/commentcorrection/2548188-7430122
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp..., http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines, http://linkedlifedata.com/resource/pubmed/chemical/Piperazines, http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor, http://linkedlifedata.com/resource/pubmed/chemical/Platelet Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Leukotriene B4, http://linkedlifedata.com/resource/pubmed/chemical/SRS-A, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/platelet activating factor receptor
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5791-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Phorbol 12-myristate 13-acetate inhibits binding of leukotriene B4 and platelet-activating factor and the responses they induce in neutrophils: site of action.
pubmed:affiliation
Department of Physiology, University of Connecticut Health Center, Farmington 06032.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't